Bioprocessing Workshop 2026

High Cell-Density Fermentation of E. coli and P. putida: From Proteins to Bioplastics

📍 Jerzy Haber Institute of Catalysis and Surface Chemistry PAS, Kraków
🗓️ June 17–19, 2026
💡 Part of Eurobiotech 2026

 

👩‍🔬 Hands-on, High-Impact, High-Cell-Density

Join us at one of Central Europe's most advanced microbiological process labs for a 3-day interactive workshop designed for biotechnologists, bioengineers, and innovators working on the frontlines of microbial production.

This workshop focuses on the design, execution, and optimization of high cell-density (HCD) fermentations for recombinant protein expression in Escherichia coli and biopolymer (PHA) biosynthesis in Pseudomonas putida using industrial-grade bioreactor systems.

 

🔬 What Will You Learn?

🧫 HCD Protein Production in E. coli – 5L & 30L Bioreactors

Learn how to boost recombinant protein yields by pushing E. coli to its physiological limits. We’ll guide you through:

• Fed-batch process design for maximizing biomass
• Induction strategies using IPTG and temperature profiles
• Monitoring DO, pH, and feeding curves in real time
• Differences in process control between bench-top (5L) and pilot (30L) scales

🌱 PHA Biopolymer Production in P. putida – 5L Fermentors

Explore how P. putida can be turned into a plastic-producing factory. Participants will:

• Operate a 5L fermentation process for PHA accumulation
• Optimize substrate feeding and nitrogen limitation
• Learn downstream biomass recovery for further PHA extraction

 

🛠️ What Will You Do?

This is not a passive seminar — it’s a true lab experience. You will:

• Assemble and sterilize bioreactors (Biostat B & C)
• Prepare and calibrate electrodes, pumps, and gas lines
• Inoculate and control high-density microbial cultures
• Initiate protein expression using IPTG
• Harvest cultures using a high-speed industrial centrifuge
• Engage in data-driven decisions based on real-time OD, DO, pH, and feeding rate curves

 

🤝 Why You Shouldn’t Miss It

• Real Fermentation Equipment – Not a demo, not a video. You'll work with actual Sartorius Biostat fermentors used in research and production.
• Cross-Scale Perspective – Compare 5L and 30L fermentations side-by-side.
• Meet the Experts – Interact with experienced researchers and engineers, including those who routinely work at the interface of academia and biotech industry.
• From Genes to Grams – Gain insights into how upstream decisions affect downstream yields.
• Workshop Certificate – Every participant receives a signed certificate confirming their practical training in HCD fermentation.

 

🎓 Who Should Attend?

• Biotech PhD students and postdocs seeking advanced bioprocessing experience
• Industrial scientists working on microbial protein or polymer production
• Fermentation technologists interested in scaling and process control
• Start-up founders and tech-transfer professionals looking to de-risk production routes
• Anyone curious about pushing bugs beyond their limits, safely and sustainably

 

🎥 Bonus Content

The workshop will be documented with professional photography and videography. You’ll get a chance to:

• See yourself in our official aftermovie
• Promote your skills and lab work on social media
• Be part of a growing community of fermentation experts!

 

🌐 Full agenda, registration details, and participant limits will be announced in early 2026.